Interplay of Conformational Plasticity and Substrate Polymorphism in Malarial tyrosyl-tRNA Synthetase
نویسندگان
چکیده
منابع مشابه
Clone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori
Aminoacyl-tRNA synthetases are the key enzymes for protein synthesis. Glycine, alanine, serine and tyrosine are the major amino acids composing fibroin of silkworm. Among them, the genes of alanyl-tRNA synthetase (AlaRS) and glycyl-tRNA synthetase (GlyRS) have been cloned. In this study, the seryl-tRNA synthetase (SerRS) and tyrosyl-tRNA synthetase (TyrRS) genes from silkworm were cloned. Their...
متن کاملCrystal structure analyses of yeast tyrosyl-tRNA synthetase
Introduction Aminoacyl-tRNA synthetases (aaRSs) play a central role in the assembly of amino acids into polypeptide chains. By selection of the correct amino acid for acylation of a specific tRNA, they provide the key for translation of the genetic code. The 20 aaRSs are divided into two classes of 10 enzymes each. TyrosyltRNA synthetase (TyrRS) is a class I enzyme, but is unusual in that it is...
متن کاملC-7 modified flavonoids as novel tyrosyl-tRNA synthetase inhibitors
Twenty C-7modified flavonoids were designed and synthesized. Biological evaluation in vitro indicated that compounds generated by SYBYL-X with high scores also showed good inhibitory activities against TyrRS. Compounds containing the nargenin core exhibit better enzyme inhibitory activities than other flavonoid cores, with (S)-5-hydroxy-40-hydroxy-7-(2-morpholino-2-oxoethoxy)-2,3-dihydroflavone...
متن کاملStructural basis for recognition of cognate tRNA by tyrosyl-tRNA synthetase from three kingdoms
The specific aminoacylation of tRNA by tyrosyl-tRNA synthetases (TyrRSs) relies on the identity determinants in the cognate tRNA(Tyr)s. We have determined the crystal structure of Saccharomyces cerevisiae TyrRS (SceTyrRS) complexed with a Tyr-AMP analog and the native tRNA(Tyr)(GPsiA). Structural information for TyrRS-tRNA(Tyr) complexes is now full-line for three kingdoms. Because the archaeal...
متن کاملtRNA-like recognition of group I introns by a tyrosyl-tRNA synthetase.
The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) functions in splicing group I introns by promoting the formation of the catalytically active RNA structure. Previous work suggested that CYT-18 recognizes a conserved tRNA-like structure of the group I intron catalytic core. Here, directed hydroxyl-radical cleavage assays show that the nucleotide-binding fold and C-ter...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.433